Development of a flow injection immunoanalysis (FIIA) for pesticide determination by means of fluorescence detection

Monika Wortberg; Cornelia Middendorf; Joerg Krause; Karl Cammann

doi:10.1117/12.144708

18 May 1993 Development of a flow injection immunoanalysis (FIIA) for pesticide determination by means of fluorescence detection

Monika Wortberg, Cornelia Middendorf, Joerg Krause, Karl Cammann

Author Affiliations +

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144708
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In this paper we describe the state of development of a flow-through immunosensor for quasi- continuous monitoring of pesticides, especially triazine herbicides, in drinking water. Starting with time-resolved fluoroimmunoassays using Eu-chelates as fluorescent labels, we obtained a detection limit of 0.1 (mu) g/l for some triazine herbicides. The experimental parameters applied in assays on microtiter strips were transferred and adjusted to small carrier beads as solid phase in affinity columns. For fluorescence detection in the FIIA a pulsed laser was used as an excitation source. The light was coupled into a flow-through cuvette and the fluorescence signal was recorded with a photomultiplier tube working in a gated detection mode.

Citation Download Citation

Monika Wortberg, Cornelia Middendorf, Joerg Krause, and Karl Cammann "Development of a flow injection immunoanalysis (FIIA) for pesticide determination by means of fluorescence detection", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144708

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