Fluorescence lifetime imaging microscopy (FLIM) and its applications

Xue Feng Wang; Ammasi Periasamy; Gerald W. Gordon; Pawel Wodnicki; Brian Herman

doi:10.1117/12.182779

17 August 1994 Fluorescence lifetime imaging microscopy (FLIM) and its applications

Xue Feng Wang, Ammasi Periasamy, Gerald W. Gordon, Pawel Wodnicki, Brian Herman

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182779
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Recent technological advances have provided an opportunity for the development of `fluorescence lifetime imaging microscopy' (FLIM). FLIM is an extremely important advance, as it allows for the first time, the sensitivity of the fluorescence lifetime to environmental parameters to be monitored in a spatial manner in single living cells. FLIM can be developed both on conventional and confocal fluorescence microscopes. Fluorescence lifetime detection can be performed using either time- or frequency-domain methods. In this paper, we report on the development of conventional and confocal FLIM systems currently underway in our laboratory. We also present three examples of current biological research projects in which we employ FLIM.

Citation Download Citation

Xue Feng Wang, Ammasi Periasamy, Gerald W. Gordon, Pawel Wodnicki, and Brian Herman "Fluorescence lifetime imaging microscopy (FLIM) and its applications", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182779

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