Shape quantification of single red blood cells based on their scattering patterns from microscopic images

Gert Schneider; Gerhard Artmann

doi:10.1117/12.200893

1 February 1995 Shape quantification of single red blood cells based on their scattering patterns from microscopic images

Gert Schneider, Gerhard Artmann

Author Affiliations +

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200893
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The differentiation between discocytic and stomatocytic red blood cell (RBC) shape using conventional microscopic imaging and image analysis tools is still on a very poor level. A procedure to differentiate the degree of stomatocytic shape changes was developed. We obtained multiple microscopic images of the same RBCs settled on a human albumin coated cover slip. The images were acquired when the microscope objective was subsequently focused through the cell layer. At equidistant horizontal planes (z-axis) below, within, and above the microscopic focal plane the light intensity distribution was considered. Using a model based on light refraction, we calculated the intensity distribution of the planes which are out of focus. Using this tool we are able to differentiate RBC shapes precisely. On the other hand, using this model from and the light intensity distributions of different focal planes, we are able to reconstruct the shape of one single RBC located in the optical axis of the microscope.

Citation Download Citation

Gert Schneider and Gerhard Artmann "Shape quantification of single red blood cells based on their scattering patterns from microscopic images", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200893

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