Dynamic light scattering study of the effect of Mg2+ and ATP on synthetic myosin filaments

Satoru Fujime; Sei-ichi Takayama

doi:10.1117/12.197462

2 January 1995 Dynamic light scattering study of the effect of Mg²⁺ and ATP on synthetic myosin filaments

Satoru Fujime, Sei-ichi Takayama

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197462
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

The dynamic light scattering (DLS) method provides us with information about the apparent diffusion coefficient, D_app, as well as the static scattering intensity, I_s. For long but thin rods with length L and diameter d (i.e., KL >> 1 but Kd << 1, K being the length of the scattering vector), the dependence on L and d of D_app is quite different from that of I_s. By means of DLS were studied synthetic myosin filaments of vertebrate skeletal muscle in solution at pH 8.3. It appeared that Mg²⁺ ions induced lengthening and thickening of the filaments whereas ATP (and ADP) induced shortening (depolymerization) of the filaments. When ATP was added to the filament preparation in the presence of Mg²⁺ ions, thinning of the filaments (or splitting into subfilaments) occurred prior to shortening (depolymerization).

Citation Download Citation

Satoru Fujime and Sei-ichi Takayama "Dynamic light scattering study of the effect of Mg²⁺ and ATP on synthetic myosin filaments", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197462

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