Electro-optical analysis of cells and cell structures

Victor D. Bunin

doi:10.1117/12.229534

8 January 1996 Electro-optical analysis of cells and cell structures

Victor D. Bunin

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229534
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

There exists two main directions of the electro-optical analysis of suspended cells. In each of these directions electrophysical properties of the cell are used. Cells in the first direction of research are considered as a whole object. Cells are influenced by electrical field, which permits us to reveal their electrophysical properties. The results of electro-optical cell suspension sample measurements are thus: (1) Determination of heterogeneity of cell population on electrophysical properties and morphometrical characteristics; (2) Determination of absolute total concentration of cells and concentration of cells in each faction with distinguished electrophysical properties. The second direction of research provides determination of electrophysical parameters of cell structures on designed homogeneous fraction. The mutual relation of a structure and physiological function of cells permits us to use the value and kinetic of electrophysical cell structure parameters change for determination of their physiological characteristics. In the manuscript the theoretical bases of each direction of the analysis and the results of their use for monitoring of biotechnological processes are considered.

Citation Download Citation

Victor D. Bunin "Electro-optical analysis of cells and cell structures", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229534

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