Three-dimensional display and quantitative analysis of multidimensional light microscope images

Lowell D. Harris; William Galbraith; Patricia A. Feineigle; D. Lansing Taylor

doi:10.1117/12.238501

15 April 1996 Three-dimensional display and quantitative analysis of multidimensional light microscope images

Lowell D. Harris, William Galbraith, Patricia A. Feineigle, D. Lansing Taylor

Proceedings Volume 2707, Medical Imaging 1996: Image Display; (1996) https://doi.org/10.1117/12.238501
Event: Medical Imaging 1996, 1996, Newport Beach, CA, United States

Abstract

Specialized high speed volume rendering tools and image preprocessing methods required for the automated interactive microscope (AIM) are described. AIM will allow the biologist to perform 'closed loop' experiments, meaning tea the biologist will be able to view preliminary results to allow critical observations which facilitate the changing of the course of image data collection dynamically during the experiment. To facilitate this process, we are developing high-speed volume rendering tools utilizing 3-D texture mapping hardware. In addition, specific preprocessing methods are described which enable the seeing of 3-D structures in volume renderings of differential interference contrast (DIC) volume images. Without these methods, structures of interest, which are critically related to the experiment, would be obscured as though in a dense fog.

Citation Download Citation

Lowell D. Harris, William Galbraith, Patricia A. Feineigle, and D. Lansing Taylor "Three-dimensional display and quantitative analysis of multidimensional light microscope images", Proc. SPIE 2707, Medical Imaging 1996: Image Display, (15 April 1996); https://doi.org/10.1117/12.238501

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