Control of the activity of an enzyme immobilized into thermoreversible hydrogel

Larisa V. Sigolaeva; Nicolay L. Eremeev; Novella F. Kazanskaya

doi:10.1117/12.237023

26 April 1996 Control of the activity of an enzyme immobilized into thermoreversible hydrogel

Larisa V. Sigolaeva, Nicolay L. Eremeev, Novella F. Kazanskaya

Proceedings Volume 2779, 3rd International Conference on Intelligent Materials and 3rd European Conference on Smart Structures and Materials; (1996) https://doi.org/10.1117/12.237023
Event: 3rd International Conference on Intelligent Materials, 1996, Lyon, France

Abstract

The effect of phase transition of a thermosensitive matrix on temperature dependencies of observed activity, equilibrium, and kinetic constants of the enzymatic reaction was studied using (alpha) -chymotrypsin immobilized into thermosensitive poly-N-isopropylacrylamide gel as a model system. The behavior of the system is determined by the properties of the enzyme at temperatures less than critical temperature corresponding to phase transition of the polymeric gel but by the properties of the thermosensitive polymeric matrix at temperatures higher than that critical temperature. The phase transition of the thermosensitive gel was shown to affect weakly temperature dependencies of equilibrium parameters of enzymatic reactions. All reversible effects of the reduction of the enzymatic activity at high temperature are exclusively due to the changes in temperature behavior of kinetic parameters of the enzymatic reaction.

Citation Download Citation

Larisa V. Sigolaeva, Nicolay L. Eremeev, and Novella F. Kazanskaya "Control of the activity of an enzyme immobilized into thermoreversible hydrogel", Proc. SPIE 2779, 3rd International Conference on Intelligent Materials and 3rd European Conference on Smart Structures and Materials, (26 April 1996); https://doi.org/10.1117/12.237023

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