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16 June 1997 Luminescence studies using high-resolution intensified digital imaging: preliminary results
James Castracane, Michelle Conerty, Lawrence P. Clow, S. Ward Casscells, David Engler
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Proceedings Volume 2976, Biomedical Sensing, Imaging, and Tracking Technologies II; (1997) https://doi.org/10.1117/12.275540
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
The use of bio-chemiluminescence immunoassay (BL/CLI) technology for molecular and cellular characterization is rapidly evolving. The excellent selectivity of this method can be exploited to identify the presence and distribution of specific cells. Current work involves the advancement of the required methods and technologies for application to the analysis of vascular wall surfaces. In this effort, various enzyme-linked antibodies are being explored which can be directed to cell surface antigens producing a luminogenic reaction. To aid in the analysis of this light emission, a custom high resolution digital imaging system which couples a multi-megapixel CCD with a specially designed image intensifier is under development. This intensifier system has high spatial resolution and excellent sensitivity in the wavelength region of the candidate BL/CL emissions. The application of this imaging system to BL/CLI requires unique performance characteristics and specialized optical design. Component level electro-optical tests of the imaging system will be presented along with design considerations for an eventual catheter based instrument. Initial in vitro experiments focused on the performance limits of the optical system in discriminating candidate luminogenic reactions. The main objective of these tests is the identification of suitable enzyme catalyzed systems for ultimate application to in vivo vascular tissue and cell diagnosis.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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James Castracane, Michelle Conerty, Lawrence P. Clow, S. Ward Casscells, and David Engler "Luminescence studies using high-resolution intensified digital imaging: preliminary results", Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); https://doi.org/10.1117/12.275540
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KEYWORDS
Imaging systems
Luminescence
Image intensifiers
Molecules
Sensors
Tissues
Charge-coupled devices
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