Bioreceptor-conducting polymer multilayer assemblies for biosensing

Lynne A. Samuelson; Shridhara Alva; Jayant Kumar; David L. Kaplan; Sukant K. Tripathy

doi:10.1117/12.305595

16 April 1998 Bioreceptor-conducting polymer multilayer assemblies for biosensing

Lynne A. Samuelson, Shridhara Alva, Jayant Kumar, David L. Kaplan, Sukant K. Tripathy

Proceedings Volume 3321, 1996 Symposium on Smart Materials, Structures, and MEMS; (1998) https://doi.org/10.1117/12.305595
Event: Smart Materials, Structures and MEMS, 1996, Bangalore, India

Abstract

This research focuses on the organized integration of biological receptors and polymers into thin film architectures for biosensing applications. Layer-by-layer electrostatic adsorption was used for the first time to form alternating protein-conducting polymer multilayers. The light-harvesting, phycobiliproteins and the enzyme, alkaline phosphatase were the bioreceptors investigated and sulfonated polystyrene, poly(diallyl dimethyl ammonium chloride) and a new enzymatically polymerized, water soluble, polyaniline were the polymer counterions used for deposition. Spectroscopic characterization was used to determine both multilayer formation and biosensing function of the final bioreceptor-polymer assemblies. These techniques have proven to be simple, chemically mild, and versatile and are expected to find application in the fabrication of ultrathin films for biosensors, opto- electronic devices and biomedical applications.

Citation Download Citation

Lynne A. Samuelson, Shridhara Alva, Jayant Kumar, David L. Kaplan, and Sukant K. Tripathy "Bioreceptor-conducting polymer multilayer assemblies for biosensing", Proc. SPIE 3321, 1996 Symposium on Smart Materials, Structures, and MEMS, (16 April 1998); https://doi.org/10.1117/12.305595

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