Formation of protoporphyrin IX in mouse skin after topical application of 5-aminolevulinic acid and its methyl esther

Roar Sorensen; Petras Juzenas; Vladimir Iani; Johan Moan

doi:10.1117/12.339121

3 February 1999 Formation of protoporphyrin IX in mouse skin after topical application of 5-aminolevulinic acid and its methyl esther

Roar Sorensen, Petras Juzenas, Vladimir Iani, Johan Moan

Author Affiliations +

Proceedings Volume 3563, Photochemotherapy of Cancer and Other Diseases; (1999) https://doi.org/10.1117/12.339121
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Normal skin of nude mice (Balb/c) was treated topically with 5-aminolevulinic acid (ALA) and its methyl ester (ALA-Me) for 24 hours. Approximately 0.1 gram of freshly prepared cream was applied to a spot of 1 cm² on the flank of the mice, which was then covered with a transparent dressing. The ALA induced protoporphyrin IX (PpIX) was studied by means of a noninvasive fiber-optic fluorescence probe connected to a luminescence spectrometer. The excitation wavelength was 407 nm, and the emission wavelength was 637 nm. For the first hour a slight lag in PpIX production was observed for the mice treated with ALA-Me compared to the mice treated with ALA. After approximately 12 hours the ALA and the ALA-Me treated mice showed the same PpIX fluorescence intensity. From 12 hours until 24 hours the PpIX fluorescence intensity decreased for both treatment modalities, even though ALA and ALA-Me were continuously present. At 24 hours ALA-Me-treated mice had less than half the amount of PpIX in their skin compared with ALA- treated mice.

Citation Download Citation

Roar Sorensen, Petras Juzenas, Vladimir Iani, and Johan Moan "Formation of protoporphyrin IX in mouse skin after topical application of 5-aminolevulinic acid and its methyl esther", Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); https://doi.org/10.1117/12.339121

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available