Real-time detection and characterization of individual flowing airborne biological particles: fluorescence spectra and elastic scattering measurements

Yongle Pan; Stephen Holler; Richard K. Chang; Steven C. Hill; Ronald G. Pinnick; Stanley Niles; Jerold R. Bottiger; Burt V. Bronk

doi:10.1117/12.371270

30 November 1999 Real-time detection and characterization of individual flowing airborne biological particles: fluorescence spectra and elastic scattering measurements

Yongle Pan, Stephen Holler, Richard K. Chang, Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Jerold R. Bottiger, Burt V. Bronk

Author Affiliations +

Proceedings Volume 3855, Air Monitoring and Detection of Chemical and Biological Agents II; (1999) https://doi.org/10.1117/12.371270
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

Real-time methods which is reagentless and could detect and partially characterize bioaerosols are of current interest. We present a technique for real-time measurement of UV-excited fluorescence spectra and two-dimensional angular optical scattering (TAOS) from individual flowing biological aerosol particles. The fluorescence spectra have been observed from more than 20 samples including Bacillus subtilis, Escherichia coli, Erwinia herbicola, allergens, dust, and smoke. The S/N and resolution of the spectra are sufficient for observing small lineshape differences among the same type of bioaerosol prepared under different conditions. The additional information from TAOS regarding particle size, shape, and granularity has the potential of aiding in distinguishing bacterial aerosols from other aerosols, such as diesel and cigarette smoke.

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Yongle Pan, Stephen Holler, Richard K. Chang, Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Jerold R. Bottiger, and Burt V. Bronk "Real-time detection and characterization of individual flowing airborne biological particles: fluorescence spectra and elastic scattering measurements", Proc. SPIE 3855, Air Monitoring and Detection of Chemical and Biological Agents II, (30 November 1999); https://doi.org/10.1117/12.371270

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