Genetically designed biosensing systems for high-throughput screening of pharmaceuticals, clinical diagnostics, and environmental monitoring

Brett Romain Wenner; Phillip Douglass; Suresh Shrestha; Bethel V. Sharma; Siyi Lai; Marc J. Madou; Sylvia Daunert

doi:10.1117/12.426736

10 May 2001 Genetically designed biosensing systems for high-throughput screening of pharmaceuticals, clinical diagnostics, and environmental monitoring

Brett Romain Wenner, Phillip Douglass, Suresh Shrestha, Bethel V. Sharma, Siyi Lai, Marc J. Madou, Sylvia Daunert

Author Affiliations +

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426736
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The genetically-modified binding proteins calmodulin, the phosphate binding protein, the sulfate binding protein, and the galactose/glucose binding protein have been successfully employed as biosensing elements for the detection of phenothiazines, phosphate, sulfate, and glucose, respectively. Mutant proteins containing unique cysteine residues were utilized in the site-specific labeling of environment-sensitive fluorescent probes. Changes in the environment of the probes upon ligand-induced conformational changes of the proteins result in changes in fluorescence intensity.

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Brett Romain Wenner, Phillip Douglass, Suresh Shrestha, Bethel V. Sharma, Siyi Lai, Marc J. Madou, and Sylvia Daunert "Genetically designed biosensing systems for high-throughput screening of pharmaceuticals, clinical diagnostics, and environmental monitoring", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426736

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