Application of two-photon microscopy to elucidate oleic-acid-induced changes in microscale transdermal transport processes

Betty Yu; Chen-Yuan Dong; Peter T. C. So; Daniel Blankschtein; Robert Langer

doi:10.1117/12.424557

24 April 2001 Application of two-photon microscopy to elucidate oleic-acid-induced changes in microscale transdermal transport processes

Betty Yu, Chen-Yuan Dong, Peter T. C. So, Daniel Blankschtein, Robert Langer

Author Affiliations +

Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424557
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

In a novel application of two-photon scanning fluorescence microscopy (TPM), three-dimensional spatial distributions of the hydrophilic and hydrophobic fluorescent probes, sulforhodamine B (SRB) and rhodamine B hexyl ester (RBHE), in excised full-thickness human cadaver skin were visualized and quantified. These findings utilizing TPM demonstrate that, in addition to providing three-dimensional images that clearly delineate probe distributions in the direction of increasing slun depth, the subsequent quantification of these images provides additional important insight into the mechanistic changes in transdermal transport underlying the visualized changes in probe distributions across the slun.

Citation Download Citation

Betty Yu, Chen-Yuan Dong, Peter T. C. So, Daniel Blankschtein, and Robert Langer "Application of two-photon microscopy to elucidate oleic-acid-induced changes in microscale transdermal transport processes", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); https://doi.org/10.1117/12.424557

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