Development of a chemiluminescence detector for analysis of nitrite in biological samples

Zhiwei Liu; Yikai Zhou; Shunqing Xu; Shu Ren; Zhiren Zhang

doi:10.1117/12.440172

14 September 2001 Development of a chemiluminescence detector for analysis of nitrite in biological samples

Zhiwei Liu, Yikai Zhou, Shunqing Xu, Shu Ren, Zhiren Zhang

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440172
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

A new instrument for the determination of low concentrations of nitrite is presented. Nitrite reacts with I^- in acidic medium to form nitric oxide that is subsequently removed from reaction solution by scrubbing with argon gas, then nitric oxide is detected by Luminol/H₂O₂ Chemiluminescence detection. The detection limit of nitrite is 10pmol for S/N equals 3. The calibration graph is linear up to 1000 pmol. The coefficient of variation for 500pmol nitrite in samples is 4.87 percent. Analysis time for single test is 250 S. The results are in good agreement with a spectrophotometric interfering ions and has been applied to the analysis of a variety of biological samples.

Citation Download Citation

Zhiwei Liu, Yikai Zhou, Shunqing Xu, Shu Ren, and Zhiren Zhang "Development of a chemiluminescence detector for analysis of nitrite in biological samples", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440172

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