Rapid drug susceptibility test of mycobacterium tuberculosis by bioluminescence sensor

Bin Lu; Shunqing Xu; Zifei Chen; Yikai Zhou

doi:10.1117/12.440173

14 September 2001 Rapid drug susceptibility test of mycobacterium tuberculosis by bioluminescence sensor

Bin Lu, Shunqing Xu, Zifei Chen, Yikai Zhou

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440173
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

With the persisting increase of drug-resistant stains of M. Tuberculosis around the world, rapid and sensitive detection of antibiotic of M. Tuberculosis is becoming more and more important. In the present study, drug susceptibility of M. tuberculosis were detected by recombination mycobacteriophage combined with bioluminescence sensor. It is based on the use of recombination mycobacteriophage which can express firefly luciferase when it infects viable mycobacteria, and can effectively produce quantifiable photon. Meanwhile, in mycobacterium cells treated with active antibiotic, no light is observed. The emitted light is recorded by a bioluminscence sensor, so the result of drug-resistant test can be determined by the naked eye. 159 stains of M. tuberculosis were applied to this test on their resistant to rifampin, streptomycin and isoniazid. It is found that the agreement of this assay with Liewenstein- Jensen slat is: rifampin 95.60 percent, isoniazid 91.82 percent, streptomycin 88.68 percent, which showed that it is a fast and practical method to scene and detect drug resistant of mycobacterium stains.

Citation Download Citation

Bin Lu, Shunqing Xu, Zifei Chen, and Yikai Zhou "Rapid drug susceptibility test of mycobacterium tuberculosis by bioluminescence sensor", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440173

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