New method to measure the contraction speed of corneal collagen under laser irradiation

Traian Dascalu; G. Cruz de Leon; M. Consolacion Martinez Saldana; C. J. Ventura Juarez

doi:10.1117/12.432904

29 June 2001 New method to measure the contraction speed of corneal collagen under laser irradiation

Traian Dascalu, G. Cruz de Leon, M. Consolacion Martinez Saldana, C. J. Ventura Juarez

Proceedings Volume 4430, ROMOPTO 2000: Sixth Conference on Optics; (2001) https://doi.org/10.1117/12.432904
Event: ROMOPTO 2000: Sixth Conference on Optics, 2000, Bucharest, Romania

Abstract

Laser thermokeratoplasty (LTK) is a very promising method to correct hyperopia and astigmatism. Thermally induced shrinkage of corneal collagen in corneal periphery with IR lasers produce an increasing of central corneal curvature. In order to increase the accuracy of changes in corneal curvature we develop the method to find out the dependence of the collagen's total contraction and contraction speed under laser irradiation. The experiments were performed on porcine eyes, in vitro, by using the fluorescence photobleaching (FP) method and CTH:YAG laser Nd:YAG laser. The collagen fibril was marked with fluorescent collagen antibody. CTH:YAG was used to produce fibril shrinkage and simultaneously an argon laser pulse produce a bleaching pattern on fluorescent marked area. By using the fluorescence microscope the signal detected by a photomultiplier is analyzed and the speed of fluorescent marker and contraction length are detected.

Citation Download Citation

Traian Dascalu, G. Cruz de Leon, M. Consolacion Martinez Saldana, and C. J. Ventura Juarez "New method to measure the contraction speed of corneal collagen under laser irradiation", Proc. SPIE 4430, ROMOPTO 2000: Sixth Conference on Optics, (29 June 2001); https://doi.org/10.1117/12.432904

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