Detection of microbial cells and preliminary estimation of their physiological state by x-ray microanalysis

Vladimir V. Sorokin; Andrei L. Mulyukin; Elena A. Vorobyova; Natalia E. Suzina; Vitalii I. Duda; Galina I. El-Registan

doi:10.1117/12.486685

30 January 2003 Detection of microbial cells and preliminary estimation of their physiological state by x-ray microanalysis

Vladimir V. Sorokin, Andrei L. Mulyukin, Elena A. Vorobyova, Natalia E. Suzina, Vitalii I. Duda, Galina I. El-Registan

Author Affiliations +

Proceedings Volume 4939, Instruments, Methods, and Missions for Astrobiology VI; (2003) https://doi.org/10.1117/12.486685
Event: Instruments, Methods, and Missions for Astrobiology V, 2002, Moscow, Russian Federation

Abstract

The method of X-ray microanalysis was used for the detection of microbial cells among cell-like particles immediately in the samples of 170 thousand year old ancient Antarctic permafrost sediments. The X-ray spectra and the quantitative parameters of P, S, Ca and K contents in cell-like particles, as well as ratios Ca/K and P/S, were compared with the corresponding data obtained for microbial cells of various physiological states: vegetative cells -- resting forms -- non-viable micromummies. The absence of P and S peaks in X-ray spectra in the most of cell-like particles allowed us to regard them as non-living objects. Among other investigated cell-like particles we were able to find the resting forms of microorganisms with the increased intracellular level of Ca, high Ca/K ratio and low P/S ratio. So, X-ray microanalysis is a promising tool for a primary detection of microbial cells in situ and determination of their physiological state.

Citation Download Citation

Vladimir V. Sorokin, Andrei L. Mulyukin, Elena A. Vorobyova, Natalia E. Suzina, Vitalii I. Duda, and Galina I. El-Registan "Detection of microbial cells and preliminary estimation of their physiological state by x-ray microanalysis", Proc. SPIE 4939, Instruments, Methods, and Missions for Astrobiology VI, (30 January 2003); https://doi.org/10.1117/12.486685

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available