Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs

Chih-kuan Tung; Tsung-Kai Chiu; Wen Lo; Po-Hsiang Wang; Sun-Jan Lin; Shiou-Hwa Jee; Chen-Yuan Dong

doi:10.1117/12.478026

10 July 2003 Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs

Chih-kuan Tung, Tsung-Kai Chiu, Wen Lo, Po-Hsiang Wang, Sun-Jan Lin, Shiou-Hwa Jee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478026
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Index mismatch induced spherical aberration is studied by comparing imaging ability of different immersion objectives in both uniformly fluorescent solutions and fluorescently labeled skin samples using scanning two-photon fluorescence microscopy. We investigated the performances of the objectives (air, water, glycerin, and oil immersion) by measuring the fluorescence profiles at different depths. In homogeneous fluorescent samples, we found that immersion medium with compatible refractive index as the samples yields better results, and small differences in refractive indices did not cause noticeable effects. Similar results were found in skin samples. Except for the air objective, we found that the choice of immersion medium did not have significant effects for in-depth imaging in the fluorescent solutions or skin samples.

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Chih-kuan Tung, Tsung-Kai Chiu, Wen Lo, Po-Hsiang Wang, Sun-Jan Lin, Shiou-Hwa Jee, and Chen-Yuan Dong "Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478026

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