Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells

Michael L. Denton; Debbie M. Eikum; Gary D. Noojin; David J. Stolarski; Randolph D. Glickman; Benjamin A. Rockwell

doi:10.1117/12.529355

1 July 2004 Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells

Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Randolph D. Glickman, Benjamin A. Rockwell

Proceedings Volume 5319, Laser Interaction with Tissue and Cells XV; (2004) https://doi.org/10.1117/12.529355
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Human retinal pigment epithelial (RPE) cells (hTERT-RPE1) were used to detect photo-oxidation products generated from chronic NIR (810 nm) laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H₂DCF-DA and its analog CM-H₂DCF-DA. Fluorescence was detected in cells exposed to mode-locked (76 MHz, ~160 femtoseconds) but not CW laser exposure. Detection of photo-oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H₂DCF-DA dye proved a more sensitive indicator of oxidation than H₂DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures (using the most sensitive fluorescent dye conditions) when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode-locked exposure.

Citation Download Citation

Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Randolph D. Glickman, and Benjamin A. Rockwell "Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); https://doi.org/10.1117/12.529355

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