Experimental and calculated enhancement factor in the SEIRA method

G. I. Dovbeshko; Yuri M. Shirshov; Vladimir I. Chegel; O. M. Fesenko

doi:10.1117/12.570019

20 July 2004 Experimental and calculated enhancement factor in the SEIRA method

G. I. Dovbeshko, Yuri M. Shirshov, Vladimir I. Chegel, O. M. Fesenko

Proceedings Volume 5507, XVI International Conference on Spectroscopy of Molecules and Crystals; (2004) https://doi.org/10.1117/12.570019
Event: XVI International Conference on Spectroscopy of Molecules and Crystals, 2003, Sevastopol, Ukraine

Abstract

The use of the method of enhancement of infrared absorption by rough metallic surface (SEIRA) allows one to increase the probability of infrared transitions and to reveal a series of spectral manifestations of structural features of biological molecules. We analyzed various experimental techniques which gives a possibility to achieve enhancement in the infrared (IR) spectra. We studied the applicability of the SEIRA techniques for analysis of nucleic acids, lipids, and membranes. Under the conditions of our experiment and according to literature data, there was observed enhancement factor equal to 3-20 for vibrations of various molecular groups. Peculiarity of another optical amplifier, namely, colloidal gold that effectively used for enhancement of signal in JR absorption and Raman scattering, have been studied. We made an attempt to model the effect of enhancement of electrical field and their frequency dependence for different metal surfaces and obtained that silver, gold and copper are the best.

Citation Download Citation

G. I. Dovbeshko, Yuri M. Shirshov, Vladimir I. Chegel, and O. M. Fesenko "Experimental and calculated enhancement factor in the SEIRA method", Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); https://doi.org/10.1117/12.570019

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