Inorganic and organic linker layers to aid immobilisation for SPR biosensing

Ian Craig; James A. McLaughlin

doi:10.1117/12.607614

2 June 2005 Inorganic and organic linker layers to aid immobilisation for SPR biosensing

Ian Craig, James A. McLaughlin

Proceedings Volume 5824, Opto-Ireland 2005: Nanotechnology and Nanophotonics; (2005) https://doi.org/10.1117/12.607614
Event: OPTO-Ireland, 2005, Dublin, Ireland

Abstract

Improvements to the immobilisation of bio-recognition elements to sensor surfaces are keenly sought. Surface Plasmon Resonance is a highly sensitive optical based sensing technique that is being used in this research as a means of evaluating novel immobilisation techniques. We report on the establishment of binding-sites at the sensor surface using two diverse methods. In the first method, well established deposition techniques were used to coat the gold surface with a silicon rich matrix. It is demonstrated that control of the depth of the material to within 10 nm was achieved. In a second method highly ordered arrays of genetically modified biological materials have been used to form attachment sites and are being investigated. Careful choice of amino acid placement at the apical domain could provide bio-selective attachment, with control in three dimensions in the region of 10's of nanometres. Characterisation of the active surfaces in each instance is presented using a number of well established techniques such as Scanning Electron Microscopy, Raman, Profilometry and Atomic Force Microscopy. Investigations, although at an early stage, have shown promise. Initial results obtained for sensitivity to glucose are indicative of an overall improvement over conventional techniques taking into account the key aspects of metal layer thickness and penetration depth of the surface plasmon wave.

Citation Download Citation

Ian Craig and James A. McLaughlin "Inorganic and organic linker layers to aid immobilisation for SPR biosensing", Proc. SPIE 5824, Opto-Ireland 2005: Nanotechnology and Nanophotonics, (2 June 2005); https://doi.org/10.1117/12.607614

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available