UV and circular dichroism thermal lens microscope for integrated chemical systems and HPLC on microchip

Kazuma Mawatari; Takehiko Kitamori

doi:10.1117/12.626456

29 September 2005 UV and circular dichroism thermal lens microscope for integrated chemical systems and HPLC on microchip

Kazuma Mawatari, Takehiko Kitamori

Author Affiliations +

Proceedings Volume 5953, Acousto-optics and Photoacoustics; 595303 (2005) https://doi.org/10.1117/12.626456
Event: Congress on Optics and Optoelectronics, 2005, Warsaw, Poland

Abstract

Thermal lens microscope (TLM) is our original sensitive detector for non-fluorescent molecules in microspace. The principle is based on absorption of light followed by photothermal process. TLM has been successfully applied tosensitive detection on microchip, and TLM enabled various applications combined with microchip technologies. We are now developing HPLC microchips as one of the important separation techniques for analysis and synthesis. For HPLC microchip systems, direct and sensitive UV detection on microchip becomes key technology. Therefore, we extended applicability of TLM from visible to UV light absorbing samples by pulse UV laser excitation (UV-TLM). Quasi- continuous wave (QCW) method was applied for lock-in amplifier detection. By applying UV-TLM for biomolecules separation and detection, about two orders of higher sensitivity was achieved compared with UV spectrophotometer. For synthesis on microchip, recognition and detection of chiral samples become important in pharmaceutical field. Therefore, function of TLM was extended for selective detection of chiral samples by utilizing polarization modulation of excitation beam and resultant circular dichroism of sample (CD-TLM). The chirality of samples was detected selectively on microchip with two orders higher sensitivity than CD spectrophotometer. Finally, we explained the instrumentation using fiber optics and micro lens technology for achieving a miniaturized practical device.

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Kazuma Mawatari and Takehiko Kitamori "UV and circular dichroism thermal lens microscope for integrated chemical systems and HPLC on microchip", Proc. SPIE 5953, Acousto-optics and Photoacoustics, 595303 (29 September 2005); https://doi.org/10.1117/12.626456

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KEYWORDS

Ultraviolet radiation

Modulation

Spectrophotometry

Laser beam diagnostics

Absorption

Microscopes

Spectroscopy

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