A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

Kyujung Kim; Donghyun Kim; Eun-jin Cho; Yong-min Huh

doi:10.1117/12.699874

14 February 2007 A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

Kyujung Kim, Donghyun Kim, Eun-jin Cho, Yong-min Huh

Author Affiliations +

Proceedings Volume 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV; 64490K (2007) https://doi.org/10.1117/12.699874
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.

Citation Download Citation

Kyujung Kim, Donghyun Kim, Eun-jin Cho, and Yong-min Huh "A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy", Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 64490K (14 February 2007); https://doi.org/10.1117/12.699874

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available