Spectroscopic and design considerations for quartz-bound Au nanoparticle SERS substrates in chemical and biological detection

William N. Radicic; Eric V. Ni; Augustus W. Fountain III

doi:10.1117/12.718730

26 April 2007 Spectroscopic and design considerations for quartz-bound Au nanoparticle SERS substrates in chemical and biological detection

William N. Radicic, Eric V. Ni, Augustus W. Fountain III

Author Affiliations +

Proceedings Volume 6554, Chemical and Biological Sensing VIII; 655407 (2007) https://doi.org/10.1117/12.718730
Event: Defense and Security Symposium, 2007, Orlando, Florida, United States

Abstract

Surface enhanced Raman spectroscopy (SERS) and spatial characterization of quartz-bound Au nanoparticle substrates has been used to assist the improvement of analytical sensitivity and limits of detection. SERS enhancement is significantly affected not only by a substrate's surface morphology but also laser-analyte orientation as well as matrix effects caused by non-analyte and non-metal substrate compounds. The use of Au hydrosols to fabricate better performing SERS substrates to detect chemical and/or biological agents has been an area of active and widespread research, but to date, the impact of matrix effects from spectral interferers introduced during fabrication on analytical sensitivity and limits of detection is not well understood. Experiments varying the depth of collection (observation) volume with respect to R6G on the substrate show high variability in analyte signal to noise ratios (S/N) well as high variability in background due to matrix effects from varying influences of the substrate non-metal components. Of the many post-fabrication design factors affecting SERS substrate sensitivity, characterization of matrix effects caused by vertical changes in observation volume near the analyte-substrate interface will improve analytical sensitivity and limits of detection.

Citation Download Citation

William N. Radicic, Eric V. Ni, and Augustus W. Fountain III "Spectroscopic and design considerations for quartz-bound Au nanoparticle SERS substrates in chemical and biological detection", Proc. SPIE 6554, Chemical and Biological Sensing VIII, 655407 (26 April 2007); https://doi.org/10.1117/12.718730

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available