Influence of glucose solution on the erythrocyte scattering properties

Elena K. Naumenko

doi:10.1117/12.725683

11 January 2007 Influence of glucose solution on the erythrocyte scattering properties

Elena K. Naumenko

Proceedings Volume 6594, Lasers for Measurements and Information Transfer 2006; 65941D (2007) https://doi.org/10.1117/12.725683
Event: Lasers for Measurements and Information Transfer 2006, 2006, St. Petersburg, Russian Federation

Abstract

The scattering characteristics of erythrocytes (the coefficients of extinction, scattering, absorption and indicatrixes) were calculated with using the theory Mie for spherical homogeneous spherical particles and the theory for two-layered spherical concentric particles. Transmission spectrums were measured with the spectrophotometer Cary500 in the wavelength range 460-860 n m. Specimens of liquid for imbedding of erythrocytes were preparing by mixing blood plasma a nd 50-% glucose solution with the different concentrations. The volume concentrations (hematocrit) of red blood cells (RBC) were maintained to have the same values in all specimens by adding equal volume of whole blood to immersion liquid of equal volumes. It has been shown that, contrary to theretical prediction, transmission is decreasing for all wavelengths with the addition of glucose solution in interval glucose volume concentrations 0.05 - 0.35-0.4. The subsequent increase of the glucose concentration leads to increasing of spectral transmission as a result of erythrocyte hemolysis.

Citation Download Citation

Elena K. Naumenko "Influence of glucose solution on the erythrocyte scattering properties", Proc. SPIE 6594, Lasers for Measurements and Information Transfer 2006, 65941D (11 January 2007); https://doi.org/10.1117/12.725683

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