Laser fluorescence fluctuation excesses in molecular immunology experiments

N. E. Galich; M. V. Filatov

doi:10.1117/12.726756

10 April 2007 Laser fluorescence fluctuation excesses in molecular immunology experiments

N. E. Galich, M. V. Filatov

Proceedings Volume 6597, Nanodesign, Technology, and Computer Simulations; 65970L (2007) https://doi.org/10.1117/12.726756
Event: Nanodesign, Technology, and Computer Simulations, 2006, Olsztyn, Poland

Abstract

A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.

Citation Download Citation

N. E. Galich and M. V. Filatov "Laser fluorescence fluctuation excesses in molecular immunology experiments", Proc. SPIE 6597, Nanodesign, Technology, and Computer Simulations, 65970L (10 April 2007); https://doi.org/10.1117/12.726756

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