Light induced changes in Raman scattering of carotenoid molecules in Photosystem I particles

Atanaska Andreeva; Silviya Abarova; Katerina Stoitchkova; Maya Velitchkova

doi:10.1117/12.727728

5 March 2007 Light induced changes in Raman scattering of carotenoid molecules in Photosystem I particles

Atanaska Andreeva, Silviya Abarova, Katerina Stoitchkova, Maya Velitchkova

Proceedings Volume 6604, 14th International School on Quantum Electronics: Laser Physics and Applications; 66042M (2007) https://doi.org/10.1117/12.727728
Event: 14th International School on Quantum Electronics: Laser Physics and Applications, 2006, Sunny Beach, Bulgaria

Abstract

The photosynthetic antenna systems are able to regulate the light energy harvesting under different light conditions by dynamic changes in their protein structure protecting the reaction center complexes. The changes modulate the electronic structure of the main antenna pigments (chlorophylls and carotenoids) and distort the characteristic planar structure of carotenoids, allowing their forbidden out of plane vibrations. Electronic absorption and low-temperature resonance Raman spectroscopy were used to study the changes in composition and spectral properties of the major carotenoids in spinach Photosystem I particles due to high light treatment. The duration of the applied intensity of the white light (1800 &mgr;E m^-2 s^-1) was 30, 60 and 120 minutes. We used Raman scattering in an attempt to recognize the type and conformation of photobleached carotenoid molecules. The resonance Raman spectra were measured at 488 and 514.5 nm, coinciding with the absorption maximum positions of the carotenoids neoxanthin and lutein, correspondingly. The results revealed nearly a full photobleaching of the long wavelength lutein molecules, whereas the bleaching of neoxantin molecules is negligible. The involvement of these changes in the photoprotection and photoinactivation of the Photosystem I particles was discussed.

Citation Download Citation

Atanaska Andreeva, Silviya Abarova, Katerina Stoitchkova, and Maya Velitchkova "Light induced changes in Raman scattering of carotenoid molecules in Photosystem I particles", Proc. SPIE 6604, 14th International School on Quantum Electronics: Laser Physics and Applications, 66042M (5 March 2007); https://doi.org/10.1117/12.727728

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KEYWORDS

Raman spectroscopy

Molecules

Particles

Absorption

Picosecond phenomena

Raman scattering

Antennas

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