Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE)

Andreas Fritz; Lars Ptaszynski; Hardo Stoehr; Ralf Brinkmann

doi:10.1117/12.728344

13 July 2007 Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE)

Andreas Fritz, Lars Ptaszynski, Hardo Stoehr, Ralf Brinkmann

Proceedings Volume 6632, Therapeutic Laser Applications and Laser-Tissue Interactions III; 66321C (2007) https://doi.org/10.1117/12.728344
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Selective Retina Treatment (SRT) is a new method to treat eye diseases associated with disorders of the RPE. Selective RPE cell damage is achieved by applying a train of 1.7 μs laser pulses at 527 nm. The treatment of retinal diseases as e.g. diabetic maculopathy (DMP), is currently investigated within clinical studies, however 200 ns pulse durations are under investigation. Transient micro bubbles in the retinal pigment epithelium (RPE) are expected to be the origin of cell damage due to irradiation with laser pulses shorter than 50 μs. The bubbles emerge at the strongly absorbing RPE melanosomes. Cell membrane disruption caused by the transient associated volume increase is expected to be the origin of the angiographically observed RPE leakage. We investigate micro bubble formation and dynamics in porcine RPE using pulse durations of 150 ns. A laser interferometry system at 830 nm with the aim of an online dosimetry control for SRT was developed. Bubble formation was detected interferometrically and by fast flash photography. A correlation to cell damage observed with a vitality stain is found. A bubble detection algorithm is presented.

Citation Download Citation

Andreas Fritz, Lars Ptaszynski, Hardo Stoehr, and Ralf Brinkmann "Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE)", Proc. SPIE 6632, Therapeutic Laser Applications and Laser-Tissue Interactions III, 66321C (13 July 2007); https://doi.org/10.1117/12.728344

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