Automated microinjection system for adherent cells

Sachihiro Youoku; Yoshinori Suto; Moritoshi Ando; Akio Ito

doi:10.1117/12.728081

13 July 2007 Automated microinjection system for adherent cells

Sachihiro Youoku, Yoshinori Suto, Moritoshi Ando, Akio Ito

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66330S (2007) https://doi.org/10.1117/12.728081
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

We have developed an automated microinjection system that can handle more than 500 cells an hour. Microinjection injects foreign agents directly into cells using a micro-capillary. It can randomly introduce agents such as DNA, proteins and drugs into various types of cells. However, conventional methods require a skilled operator and suffer from low throughput. The new automated microinjection techniques we have developed consist of a Petri dish height measuring method and a capillary apex position measuring method. The dish surface height is measured by analyzing the images of cells that adhere to the dish surface. The contrast between the cell images is minimized when the focus plane of an object lens coincides with the dish surface. We have developed an optimized focus searching method with a height accuracy of ±0.2 um. The capillary apex position detection method consists of three steps: rough, middle, and precise. These steps are employed sequentially to cover capillary displacements of up to ±2 mm, and to ultimately accomplish an alignment accuracy of less than one micron. Experimental results using this system we developed show that it can introduce fluorescent material (Alexa488) into adherent cells, HEK293, with a success rate of 88.5%.

Citation Download Citation

Sachihiro Youoku, Yoshinori Suto, Moritoshi Ando, and Akio Ito "Automated microinjection system for adherent cells", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330S (13 July 2007); https://doi.org/10.1117/12.728081

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