Fast and reliable identification of microorganisms by means of Raman spectroscopy

Petra Rösch; Michaela Harz; Mario Krause; Jürgen Popp

doi:10.1117/12.728114

13 July 2007 Fast and reliable identification of microorganisms by means of Raman spectroscopy

Petra Rösch, Michaela Harz, Mario Krause, Jürgen Popp

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66331A (2007) https://doi.org/10.1117/12.728114
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

The identification of bacteria is necessary as fast as possible e.g. to provide an appropriate therapy for patients. Here the cultivation time should be kept to a minimum. Beside microbiological identification methods Raman spectroscopy is a valuable tool for bacteria identification. UV-resonance Raman spectroscopy enables selective monitoring of the cellular DNA/RNA content and allows for a genotaxonomic classification of the bacteria. Since UV excitation may lead to sample destruction the measurements are performed on rotated bacterial films. For a faster identification avoiding the cultivation step single bacteria analysis is necessary. Using micro-Raman spectroscopy a spatial resolution in the size range of the bacteria can be achieved. With this Raman excitation the chemical components of the whole cell are measured which leads to a phenotypic classification. For localization of bacteria inside complex matrices fluorescence labeling is achieved.

Citation Download Citation

Petra Rösch, Michaela Harz, Mario Krause, and Jürgen Popp "Fast and reliable identification of microorganisms by means of Raman spectroscopy", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331A (13 July 2007); https://doi.org/10.1117/12.728114

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