A sandwich assay for procalcitonin detection for POCT applications

Francesco Baldini; Luca Bolzoni; Ambra Giannetti; Melanie Kess; Petra M. Kraemer; Elisabeth Kremmer; Giampiero Porro; Folco Senesi; Cosimo Trono

doi:10.1117/12.810265

23 February 2009 A sandwich assay for procalcitonin detection for POCT applications

Francesco Baldini, Luca Bolzoni, Ambra Giannetti, Melanie Kess, Petra M. Kraemer, Elisabeth Kremmer, Giampiero Porro, Folco Senesi, Cosimo Trono

Author Affiliations +

Proceedings Volume 7169, Advanced Biomedical and Clinical Diagnostic Systems VII; 716905 (2009) https://doi.org/10.1117/12.810265
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A plastic biochip was developed for the detection of procalcitonin (PCT) and consists of a polymethylmethacrylate (PMMA) chip shaped in order to achieve several flow microchannels. A sandwich assay using a new antibody pairs is implemented with the capture antibody immobilized on the PMMA surface and the target antibody labelled with a fluorophore. A laser diode excites the fluorescent sensing layer. Thanks to the anisotropy of the fluorescence the emitted light travels along the thickness of the plastic material. The fluorescence coming out from the chip is collected by 1 mm plastic optical fibre and detected with a spectrum analyser.

Citation Download Citation

Francesco Baldini, Luca Bolzoni, Ambra Giannetti, Melanie Kess, Petra M. Kraemer, Elisabeth Kremmer, Giampiero Porro, Folco Senesi, and Cosimo Trono "A sandwich assay for procalcitonin detection for POCT applications", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716905 (23 February 2009); https://doi.org/10.1117/12.810265

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available