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In the PDT practice for tumor patients, the dose and irradiation time for the treatment are chosen by experience and not
by real need. To establish advanced PDD-PDT model system for patients, we developed a method for monitoring the
cell-death based on a spectrophotometric real-time change in fluorescence in HeLa-tumors during Photofrin®-PDT and
ALA-PDT. Here, we describe the results of application of the new PDD-PDT system to human tumors. The fluorescence
spectra obtained from human tumors were analyzed by the differential spectral analysis. The mass-spectral changes of
tumor tissues during PDD-PDT were also examined by MALDI-TOF-MS/MS. The first author's seborrheic keratosis was
monitored with this system during the PDD-PDT with a topically applied ALA-ointment. The changes in fluorescence
spectrum were successfully detected, and the tumor regressed completely within 5 months. The differential spectral
analysis of PDD-PDT-fluorescence monitoring spectra of tumors and isolated mitochondria showed a marked decrease of
three peaks in the red region indicative of the PDD (600 - 720 nm), and a transient rise followed by a decline of peaks in
the green region indicative of the PDT (450 - 580 nm). The MALDI-TOF-MS analysis of PDD-PDT HeLa-tumors
showed a consumption of Photofrin-deuteroporphyrin and ALA-PpIX, and decreases in protein mass in the range of
4,000 - 16,000 Da, m/z 4929, 8564, 10089, 15000, and an increase in m/z 7002 in a Photofrin® PDD-PDT monitoring
tumor.
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