Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water

Mayu Otani; Kunihiro Sato

doi:10.1117/12.906313

2 February 2012 Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water

Mayu Otani, Kunihiro Sato

Author Affiliations +

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 82270T (2012) https://doi.org/10.1117/12.906313
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

A lens-less holographic microscopy is developed for observation of high-resolution 4-D (spatio-temporal) images. A complex-amplitude in-line hologram with a large numerical aperture is extracted from a large off-axis hologram by applying one-shot digital holography. An angular spectrum method is developed for fast numerical reconstruction of precise 3-D image from the in-line hologram with a large numerical aperture. 3-D intensity and phase images with no distortion, with a resolution higher than 1μm, and with a depth larger than 1mm are recorded and reconstructed by the present holographic microscopy. Possibility is demonstrated to realize 4-D measurement of microorganisms swimming in water.

Citation Download Citation

Mayu Otani and Kunihiro Sato "Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82270T (2 February 2012); https://doi.org/10.1117/12.906313

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