Development of a compact bio-optofluidic cell sorter

Andrew Banas; Darwin Palima; Finn Pedersen; Jesper Glückstad

doi:10.1117/12.909908

10 February 2012 Development of a compact bio-optofluidic cell sorter

Andrew Banas, Darwin Palima, Finn Pedersen, Jesper Glückstad

Proceedings Volume 8274, Complex Light and Optical Forces VI; 82740N (2012) https://doi.org/10.1117/12.909908
Event: SPIE OPTO, 2012, San Francisco, California, United States

Abstract

We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.

Citation Download Citation

Andrew Banas, Darwin Palima, Finn Pedersen, and Jesper Glückstad "Development of a compact bio-optofluidic cell sorter", Proc. SPIE 8274, Complex Light and Optical Forces VI, 82740N (10 February 2012); https://doi.org/10.1117/12.909908

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available