Combining large area fluorescence with multiphoton microscopy for improved detection of oral epithelial neoplasia
(Conference Presentation)

Rahul Pal; Jinping Yang; Suimin Qiu; Susan McCammon; Vicente Resto; Gracie Vargas

doi:10.1117/12.2213927

3 May 2016 Combining large area fluorescence with multiphoton microscopy for improved detection of oral epithelial neoplasia (Conference Presentation)

Rahul Pal, Jinping Yang, Suimin Qiu, Susan McCammon, Vicente Resto, Gracie Vargas

Author Affiliations +

Proceedings Volume 9701, Multimodal Biomedical Imaging XI; 970109 (2016) https://doi.org/10.1117/12.2213927
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Volumetric Multiphoton Autofluorescence Microscopy (MPAM) and Second Harmonic Generation Microscopy (SHGM) show promise for revealing indicators of neoplasia representing the complex microstructural organization of mucosa, potentially providing high specificity for detection of neoplasia, but is limited by small imaging area. Large area fluorescence methods on the other hand show high sensitivity appropriate for screening but are hampered by low specificity. In this study, we apply MPAM-SHGM following guidance from large area fluorescence, by either autofluorescence or a targeted metabolic fluorophore, as a potentially clinically viable approach for detection of oral neoplasia. Sites of high neoplastic potentially were identified by large area red/green autofluorescence or by a fluorescently labelled deoxy-glucose analog, 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2-NBDG) to highlight areas of high glucose uptake across the buccal pouch of a hamster model for OSCC. Follow-up MPAM-SHGM was conducted on regions of interests (ROIs) to assess whether microscopy would reveal microscopic features associated with neoplasia to confirm or exclude large area fluorescence findings. Parameters for analysis included cytologic metrics, 3D epithelial connective tissue interface metrics (MPAM-SHGM) and intensity of fluorescence (widefield). Imaged sites were biopsied and processed for histology and graded by a pathologist. A small sample of human ex vivo tissues were also imaged. A generalized linear model combining image metrics from large area fluorescence and volumetric MPAM-SHGM indicated the ability to delineate normal and inflammation from neoplasia.

Conference Presentation

Citation Download Citation

Rahul Pal, Jinping Yang, Suimin Qiu, Susan McCammon, Vicente Resto, and Gracie Vargas "Combining large area fluorescence with multiphoton microscopy for improved detection of oral epithelial neoplasia (Conference Presentation)", Proc. SPIE 9701, Multimodal Biomedical Imaging XI, 970109 (3 May 2016); https://doi.org/10.1117/12.2213927

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Luminescence

Multiphoton microscopy

Microscopy

3D modeling

Analog electronics

Connective tissue

Glucose

Show All Keywords

Keywords/Phrases

Search In:

Publication Years