Mesoscopic Oblique Plane Microscopy (MOPM) with near-isotropic resolutions by utilizing diffractive light sheet illumination

Wenjun Shao; Ji Yi

doi:10.1117/12.2610506

2 March 2022 Mesoscopic Oblique Plane Microscopy (MOPM) with near-isotropic resolutions by utilizing diffractive light sheet illumination

Wenjun Shao, Ji Yi

Author Affiliations +

Proceedings Volume PC11971, High-Speed Biomedical Imaging and Spectroscopy VII; PC119710G (2022) https://doi.org/10.1117/12.2610506
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We created a highly-tilted light sheet in oblique plane microscopy (OPM) by using a transmission grating such that the angle of illumination does not rely on the numerical aperture (NA) of the primary objective lens. By decoupling the light sheet angle from the NA, MOPM breaks the diffraction-limited resolution in the axial direction imposed by the NA of the primary lens. Centimeter-scale field-of-view and near-isotropic resolutions can be achieved in OPM by utilizing low NA objective lens. Imaging speed of 2 volumes per second (VPS) and 12 VPS has been demonstrated in imaging the neurons and heartbeat in whole zebrafish.

Conference Presentation

Citation Download Citation

Wenjun Shao and Ji Yi "Mesoscopic Oblique Plane Microscopy (MOPM) with near-isotropic resolutions by utilizing diffractive light sheet illumination", Proc. SPIE PC11971, High-Speed Biomedical Imaging and Spectroscopy VII, PC119710G (2 March 2022); https://doi.org/10.1117/12.2610506

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