Intravital optical imaging of wound healing in human skin (Conference Presentation)

Mihaela Balu; Juvinch Vicente; Amanda Durkin; Kristen M. Kelly; Jessica Shiu; Anand K. Ganesan

doi:10.1117/12.2648732

15 March 2023 Intravital optical imaging of wound healing in human skin (Conference Presentation)

Mihaela Balu, Juvinch Vicente, Amanda Durkin, Kristen M. Kelly, Jessica Shiu, Anand K. Ganesan

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC1238404 (2023) https://doi.org/10.1117/12.2648732
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Wound re-epithelialization is complex and imperfect. Understanding which cells are involved in this process, how they are spatially arranged, and when they contribute to wound healing is a longstanding scientific and clinical challenge. We used a recently developed fast large area multiphoton exoscope for in vivo imaging of human skin to study the process of wound healing in vivo in human skin. We monitored the re-epithelization of wounds generated by removal of the epidermis following a suction blistering procedure and identified the morphological and metabolic signatures of epidermal and dermal cells involved in the healing process.

Conference Presentation

Citation Download Citation

Mihaela Balu, Juvinch Vicente, Amanda Durkin, Kristen M. Kelly, Jessica Shiu, and Anand K. Ganesan "Intravital optical imaging of wound healing in human skin (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC1238404 (15 March 2023); https://doi.org/10.1117/12.2648732

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KEYWORDS

Wound healing

Skin

Optical imaging

In vivo imaging

Optical tracking

Optical fibers

Resolution enhancement technologies

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