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15 March 2023 Quantitative, reproducible Fluorescence Lifetime Imaging made easy (Conference Presentation)
Felix Koberling
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Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840E (2023) https://doi.org/10.1117/12.2655591
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Fluorescence Lifetime Imaging (FLIM) has become more attractive in recent years as it offers increased specificity in many assays as well as the possibility of multiplexing the read out of many markers with a small number of detectors. Here we present how FLIM modalities are implemented in Luminosa, the new single-photon counting confocal microscope by PicoQuant. Thanks to a dynamic binding format and GPU-based algorithms FLIM images of 1024x1024 can be analyzed in a few seconds. The FLIM analysis workflow suggests the best fitting model based on statistical arguments and requires minimal user interaction making these modalities become accessible to new users who can then confidently start working with FLIM and incorporate it into their research toolbox combining the strengths of phasor plots with decay fitting.
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Felix Koberling "Quantitative, reproducible Fluorescence Lifetime Imaging made easy (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840E (15 March 2023); https://doi.org/10.1117/12.2655591
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KEYWORDS
Fluorescence lifetime imaging
Statistical analysis
Confocal microscopy
Microscopes
Microscopy
Multiplexing
Process modeling
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