Vibrational tags for Raman and infrared-based imaging (Conference Presentation)

Yong Li; Katherine Townsend; Robert Dorn; Jennifer Prescher; Eric O. Potma

doi:10.1117/12.2654603

15 March 2023 Vibrational tags for Raman and infrared-based imaging (Conference Presentation)

Yong Li, Katherine Townsend, Robert Dorn, Jennifer Prescher, Eric O. Potma

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239209 (2023) https://doi.org/10.1117/12.2654603
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Recent developments have shown the utility of vibrational tags in both Raman-based microscopy and infrared-based microspectroscopy. In this context, the availability of probes that feature a strong response is highly desirable. Here we develop vibrational tags that have strong and narrow vibrational lines in the cell silent region of the spectrum. We screened numerous chemical motifs with density functional theory (DFT). Design criteria included a distinct resonance frequency, a long vibrational lifetime, and overall strength of the vibrational response. We discuss the utility of these optimized probes for cellular imaging studies with stimulated Raman scattering and Fourier transform infrared microspectroscopy.

Conference Presentation

Citation Download Citation

Yong Li, Katherine Townsend, Robert Dorn, Jennifer Prescher, and Eric O. Potma "Vibrational tags for Raman and infrared-based imaging (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239209 (15 March 2023); https://doi.org/10.1117/12.2654603

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available