Studying light-matter interaction at the nanoscale by single-molecule fluorescence lifetime imaging (smFLIM)

Margoth Córdova-Castro; Clément Cabriel; Yannick De Wilde; Valentina Krachmalnicoff; Ignacio Izeddin

doi:10.1117/12.2649961

17 March 2023 Studying light-matter interaction at the nanoscale by single-molecule fluorescence lifetime imaging (smFLIM)

Margoth Córdova-Castro, Clément Cabriel, Yannick De Wilde, Valentina Krachmalnicoff, Ignacio Izeddin

Author Affiliations +

Proceedings Volume PC12430, Quantum Sensing and Nano Electronics and Photonics XIX; PC1243013 (2023) https://doi.org/10.1117/12.2649961
Event: SPIE OPTO, 2023, San Francisco, California, United States

Abstract

In this contribution, we will report on a novel technique recently developed at Institut Langevin that enables multiplexed and super-resolved fluorescence lifetime imaging at the single-molecule level (smFLIM) with a field of view of ~10 µm2 and a localization precision of ~15 nm. Our method combines the use of an EMCCD camera for the localization of photoactivatable single molecules (as usually done in SMLM) and a linear array of single-photon avalanche diodes (SPADs) for time-resolved measurements. Our method can be used for LDOS imaging of disordered and deterministic structures with nanometer-sized features and extensions going from a few hundred nanometers to several microns, with a dynamic temporal range spanning from ns to ms. We will show how smFLIM allows the study of simple nanostructures such as a silver nanowire and complex nanostructures, such as periodic arrays of hollow gold truncated nanocones (nanochimneys).

Conference Presentation

Citation Download Citation

Margoth Córdova-Castro, Clément Cabriel, Yannick De Wilde, Valentina Krachmalnicoff, and Ignacio Izeddin "Studying light-matter interaction at the nanoscale by single-molecule fluorescence lifetime imaging (smFLIM)", Proc. SPIE PC12430, Quantum Sensing and Nano Electronics and Photonics XIX, PC1243013 (17 March 2023); https://doi.org/10.1117/12.2649961

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KEYWORDS

Light-matter interactions

Fluorescence lifetime imaging

Nanostructuring

Nanophotonics

Biophysics

Dielectrics

Imaging systems

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