Force-activated DNA substrates for single-molecule studies

Arnulf Taylor; Stephen Okoniewski; Lyle Uyetake; Thomas T. Perkins

doi:10.1117/12.2676840

5 October 2023 Force-activated DNA substrates for single-molecule studies

Arnulf Taylor, Stephen Okoniewski, Lyle Uyetake, Thomas T. Perkins

Proceedings Volume PC12649, Optical Trapping and Optical Micromanipulation XX; PC1264907 (2023) https://doi.org/10.1117/12.2676840
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

Optical traps are widely used to study nucleic-acid-processing enzymes. To investigate these enzymes, we developed “force-activated” DNA substrates that contain a pair of nicks, allowing displacement of single-stranded DNA when pulled into DNA’s overstretching transition. We designed these substrates to include DNA hairpins and are using them to investigate the mechanism of E. coli RecQ helicase, an enzyme that unwinds double-stranded DNA. Force-activated DNA substrates also have the potential to provide an easy-to-use intrinsic force standard at ~15 pN, suitable for all three major force spectroscopy modalities (i.e., optical traps, magnetic tweezers, and AFM).

Conference Presentation

Citation Download Citation

Arnulf Taylor, Stephen Okoniewski, Lyle Uyetake, and Thomas T. Perkins "Force-activated DNA substrates for single-molecule studies", Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC1264907 (5 October 2023); https://doi.org/10.1117/12.2676840

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