Chromatic focal shift calibration for confocal corrected attenuation coefficient imaging

Johannes Kübler; Vincent S. Zoutenbier; Gijs Buist; Jörg Fischer; Arjen Amelink; Johannes F. de Boer

doi:10.1117/12.3005658

13 March 2024 Chromatic focal shift calibration for confocal corrected attenuation coefficient imaging

Johannes Kübler, Vincent S. Zoutenbier, Gijs Buist, Jörg Fischer, Arjen Amelink, Johannes F. de Boer

Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC128301Y (2024) https://doi.org/10.1117/12.3005658
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Calibrating intensity values of optical coherence tomography (OCT) images helps to extract valuable tissue information, such as the attenuation coefficient. To achieve this, knowledge of focus position and Rayleigh length is crucial. We study the potential of obtaining the focus position from an OCT scan acquired with a single focus setting using the system’s longitudinal chromatic aberration. Different focus positions for sub-spectrum reconstructed OCT images are realized by taking advantage of the chromatic focal shift. Ratios of these sub-spectral images are used to estimate the focus position. High-resolution B-scan reconstruction is demonstrated by coherently combining sub-spectrum confocal function corrected B-scans, followed by OCT attenuation coefficient imaging. Additionally, we present an approach to experimentally identify the chromatic focal shift from OCT data itself.

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Johannes Kübler, Vincent S. Zoutenbier, Gijs Buist, Jörg Fischer, Arjen Amelink, and Johannes F. de Boer "Chromatic focal shift calibration for confocal corrected attenuation coefficient imaging", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC128301Y (13 March 2024); https://doi.org/10.1117/12.3005658

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KEYWORDS

Optical coherence tomography

Attenuation

Colorimetry

Calibration

Confocal microscopy

Image restoration

Optical calibration

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