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13 March 2024 Non-invasive and noise-robust confocal wavefront shaping
Dror Aizik, Anat Levin
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Proceedings Volume PC12851, Adaptive Optics and Wavefront Control for Biological Systems X; PC1285104 (2024) https://doi.org/10.1117/12.3000340
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Wavefront shaping attempts to image deep inside scattering tissue by modulating the incoming and/or outgoing wavefronts. Previously such modulations were estimated using non-linear two photon fluorescent feedback. Alternatively, to use a simpler linear fluorescent feedback, strong fluorescent beads are manually added to the tissue. However, the fluorescent components of a tissue are order-of-magnitude weaker than such beads, and the feedback signal required for previous algorithms cannot be detected. We suggest a new, noise robust approach, which works with a confocal modulation of both the illumination and imaging arms. We successfully use it to image EGFP labeled neurons through scattering tissue.
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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Dror Aizik and Anat Levin "Non-invasive and noise-robust confocal wavefront shaping", Proc. SPIE PC12851, Adaptive Optics and Wavefront Control for Biological Systems X, PC1285104 (13 March 2024); https://doi.org/10.1117/12.3000340
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KEYWORDS
Wavefronts
Confocal microscopy
Modulation
Tissues
Image sensors
Light sources and illumination
Photons
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