Determination of multiple analytes using a fiber optic biosensor based on fluorescence energy transfer

Richard B. Thompson; Zhengfang Ge; Marcia W. Patchan; Carol A. Fierke; Keith A. McCall; Daniel Elbaum; David W. Christianson

doi:10.1117/12.237624

1 April 1996 Determination of multiple analytes using a fiber optic biosensor based on fluorescence energy transfer

Richard B. Thompson, Zhengfang Ge, Marcia W. Patchan, Carol A. Fierke, Keith A. McCall, Daniel Elbaum, David W. Christianson

Author Affiliations +

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237624
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Recently, we have developed a biosensor for zinc based on the very tight binding of this metal by the enzyme carbonic anhydrase, which requires Zn(II) for catalysis. We were able to transduce the binding of the metal as a change in fluorescence intensity or lifetime by use of a colored inhibitor whose metal-dependent binding permits fluorescence resonance energy transfer (Forster transfer) to occur. We have extended this concept to include other metals and other analytes which may be bound in the native (or mutant) enzyme active site with a concomitant color change; the color change is transduced as a change in energy transfer efficiency. We have also recently demonstrated a similar approach, wherein the presence of a metal ion in the binding site is transduced as a change in fluorescence anisotropy. Results in cuvettes and with fiber optic sensors are shown.

Citation Download Citation

Richard B. Thompson, Zhengfang Ge, Marcia W. Patchan, Carol A. Fierke, Keith A. McCall, Daniel Elbaum, and David W. Christianson "Determination of multiple analytes using a fiber optic biosensor based on fluorescence energy transfer", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237624

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