Dr. Richard B. Thompson Profile

Dr. Richard B. Thompson

Associate Professor at Univ of Maryland School of Medicine

SPIE Involvement:

Author

Publications (28)

SPIE Journal Paper | 21 April 2020 Open Access

Imaging hydroxyapatite in sub-retinal pigment epithelial deposits by fluorescence lifetime imaging microscopy with tetracycline staining

Henryk Szmacinski, Kavita Hegde, Hui-Hui Zeng, Katayoun Eslami, Adam Puche, Imre Lengyel, Richard B. Thompson

JBO, Vol. 25, Issue 04, 047001, (April 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.4.047001

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Modulation, Retina, Phase shift keying, Microscopy, Tissues, Bone, Eye, Data modeling

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Proceedings Article | 12 February 2018 Presentation + Paper

Towards early detection of age-related macular degeneration with tetracyclines and FLIM

Henryk Szmacinski, Kavita Hegde, Hui-Hui Zeng, Katayoun Eslami, Adam Puche, Joseph Lakowicz, Imre Lengyel, Richard Thompson

Proceedings Volume 10484, 1048409 (2018) https://doi.org/10.1117/12.2303768

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Retina, Modulation, Microscopy, Eye, In vivo imaging, Data modeling

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SPIE Journal Paper | 1 August 2011 Open Access

Genetically encoded ratiometric biosensors to measure intracellular exchangeable zinc in Escherichia coli

Da Wang, Tamika Hurst, Carol Fierke, Richard Thompson

JBO, Vol. 16, Issue 08, 087011, (August 2011) https://doi.org/10.1117/12.10.1117/1.3613926

KEYWORDS: Zinc, Sensors, Fluorescence resonance energy transfer, Luminescence, Double positive medium, Calibration, Proteins, In vitro testing, Fluorescent proteins, Biosensors

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Proceedings Article | 1 April 2005 Paper

In situ measurement of free zinc in an ischemia model and cell culture using a ratiometric fluorescence-based biosensor

Hui-Hui Zeng, Rebecca Bozym, Robert Rosenthal, Gary Fiskum, Cynthia Cotto-Cumba, Nissa Westerberg, Carol Fierke, Andrea Stoddard, Michele Cramer, Christopher Frederickson, Richard Thompson

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.614168

KEYWORDS: Zinc, Ions, Biosensors, Brain, Ischemia, In situ metrology, Animal model studies, Proteins, Fiber optics tests, Fiber optics

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Proceedings Article | 1 July 2004 Paper

In vivo and intracellular sensing and imaging of free zinc ion

Rebecca Bozym, Hui-Hui Zeng, Michele Cramer, Andrea Stoddard, Carol Fierke, Richard Thompson

Proceedings Volume 5318, (2004) https://doi.org/10.1117/12.542329

KEYWORDS: Zinc, Proteins, Ions, Fiber optics sensors, Sensors, Brain, Calibration, Ischemia, In vivo imaging, Metals

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SPIE Journal Paper | 1 October 2002 Open Access

Excitation ratiometric fluorescent biosensor for zinc ion at picomolar levels

Richard Thompson, Michele Cramer, Rebecca Bozym, Carol Fierke

JBO, Vol. 7, Issue 04, (October 2002) https://doi.org/10.1117/12.10.1117/1.1501886

KEYWORDS: Zinc, Luminescence, Ions, Proteins, Calcium, Energy transfer, Biosensors, Metals, Biology, Fluorescence resonance energy transfer

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SPIE Journal Paper | 1 October 2002 Open Access

Tools for Biomolecular and Cellular Analysis

Fran Ligler, Richard Thompson

JBO, Vol. 7, Issue 04, (October 2002) https://doi.org/10.1117/12.10.1117/1.1513562

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Proceedings Article | 4 June 2002 Paper

Real-time in-situ determination of free Cu(II) at picomolar levels in sea water using a fluorescence lifetime-based fiber optic biosensor

Richard Thompson, Hui-Hui Zeng, Carol Fierke, Gary Fones, James Moffett

Proceedings Volume 4625, (2002) https://doi.org/10.1117/12.469783

KEYWORDS: Chemical analysis, Luminescence, Sensors, Ions, Copper, Modulation, Phase shift keying, Fiber optics, Biosensors, Optical fibers

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Proceedings Article | 22 May 2001 Paper

Ratiometric fluorescence imaging of free Zn2+ in brain

Richard Thompson, Sang Suh, Christopher Frederickson

Proceedings Volume 4255, (2001) https://doi.org/10.1117/12.426749

KEYWORDS: Zinc, Luminescence, Ions, Brain, Neuroimaging, Calcium, Microscopes, Metals, Biosensing, Proteins

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Proceedings Article | 10 May 2001 Paper

Real-time quantitation of Cu(II) by a fluorescence-based biosensing approach

Richard Thompson, Hui-Hui Zeng, Badri Maliwal, Carol Fierke

Proceedings Volume 4252, (2001) https://doi.org/10.1117/12.426733

KEYWORDS: Copper, Metals, Ions, Luminescence, Sensors, Biosensing, Proteins, Optical fibers, Zinc, Phase measurement

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Proceedings Article | 11 April 2000 Paper

Issues in enzyme-based metal ion biosensing in complex media

Richard Thompson, Hui-Hui Zeng, Michele Loetz, Carol Fierke

Proceedings Volume 3913, (2000) https://doi.org/10.1117/12.382025

KEYWORDS: Ions, Metals, Proteins, Copper, Transducers, Biosensors, Sensors, Zinc, Molecules, Luminescence

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SPIE Journal Paper | 1 January 2000 Open Access

Zinc biosensing with multiphoton excitation using carbonic anhydrase and improved fluorophores

Richard Thompson, Badri Maliwal, Hui-Hui Zeng

JBO, Vol. 5, Issue 01, (January 2000) https://doi.org/10.1117/12.10.1117/1.429963

KEYWORDS: Zinc, Anisotropy, Luminescence, Ions, Biosensing, Fluorescence anisotropy, Two photon excitation microscopy, Quantum efficiency, Proteins, Modulation

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Proceedings Article | 15 December 1999 Paper

Improved response of a fluorescence-based metal ion biosensor using engineered carbonic anhydrase variants

Richard Thompson, Hui-Hui Zeng, Michele Loetz, Keith McCall, Carol Fierke

Proceedings Volume 3858, (1999) https://doi.org/10.1117/12.372913

KEYWORDS: Ions, Metals, Copper, Biosensors, Proteins, Transducers, Luminescence, Zinc, Sensors, Biological research

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Proceedings Article | 3 May 1999 Paper

Fluorescence-based sensing of transition metal ions by a carbonic anhydrase transducer with a tethered fluorophore

Richard Thompson, Badri Maliwal, Carol Fierke

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347524

KEYWORDS: Ions, Metals, Luminescence, Modulation, Anisotropy, Proteins, Transition metals, Transducers, Fluorescence anisotropy, Phase shift keying

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Proceedings Article | 21 April 1999 Paper

Improved fluorophores for zinc biosensing using carbonic anhydrase

Richard Thompson, Badri Maliwal, Hui-Hui Zeng

Proceedings Volume 3603, (1999) https://doi.org/10.1117/12.346738

KEYWORDS: Zinc, Luminescence, Anisotropy, Biosensing, Ions, Absorbance, Fluorescence anisotropy, Molecules, Metals, Proteins

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Proceedings Article | 1 May 1998 Paper

Determination of metal ions by fluorescence anisotropy exhibits a broad dynamic range

Richard Thompson, Badri Maliwal, Carol Fierke

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307077

KEYWORDS: Metals, Ions, Zinc, Anisotropy, Fluorescence anisotropy, Luminescence, Calcium, Sensors, Polarization, Microscopes

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Proceedings Article | 10 April 1998 Paper

High-sensitivity determination of Zn(II) and Cu(II) in vitro by fluorescence polarization

Richard Thompson, Badri Maliwal, Vincent Feliccia, Carol Fierke

Proceedings Volume 3259, (1998) https://doi.org/10.1117/12.307337

KEYWORDS: Luminescence, Anisotropy, Ions, Polarization, Metals, Zinc, Fluorescence anisotropy, Molecules, In vitro testing, Biosensing

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Proceedings Article | 7 May 1997 Paper

Fluorescence-lifetime-based determination of anions using a site-directed mutant enzyme transducer

Richard Thompson, Hai-Jui Lin, Zhengfang Ge, Kelly Johnson, Carol Fierke

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273524

KEYWORDS: Metals, Ions, Absorbance, Luminescence, Cyanide, Energy transfer, Fluorescent markers, Phase shift keying, Modulation, Sensors

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Proceedings Article | 1 April 1996 Paper

Determination of multiple analytes using a fiber optic biosensor based on fluorescence energy transfer

Richard Thompson, Zhengfang Ge, Marcia Patchan, Carol Fierke, Keith McCall, Daniel Elbaum, David Christianson

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237624

KEYWORDS: Metals, Ions, Luminescence, Energy transfer, Biosensors, Fiber optics, Fluorescence anisotropy, Anisotropy, Biological research, Fiber optics sensors

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SPIE Journal Paper | 2 January 1996 Open Access

Performance enhancement of fluorescence energy transfer-based biosensors by site-directed mutagenesis of the transducer

Richard Thompson, Zhengfang Ge, Marcia Patchan, Carol Fierke

JBO, Vol. 1, Issue 01, (January 1996) https://doi.org/10.1117/12.10.1117/12.227108

KEYWORDS: Zinc, Energy transfer, Phase shift keying, Biosensors, Modulation, Luminescence, Transducers, Ions, Energy efficiency, Proteins

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Proceedings Article | 29 September 1995 Paper

Site-specific mutants of carbonic anhydrase for fluorescence energy-transfer-based metal-ion biosensing

Richard Thompson, Zhengfang Ge, Marcia Patchan, Laura Kiefer, Carol Fierke

Proceedings Volume 2508, (1995) https://doi.org/10.1117/12.221726

KEYWORDS: Zinc, Metals, Luminescence, Ions, Energy transfer, Phase shift keying, Modulation, Fluorescent markers, Absorbance, Biosensors

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Proceedings Article | 8 May 1995 Paper

Energy-transfer-based fiber optic metal-ion biosensor

Richard Thompson, Zhengfang Ge, Marcia Patchan, Carol Fierke

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208473

KEYWORDS: Zinc, Biosensors, Fiber optics, Energy transfer, Metals, Luminescence, Ions, Semiconductor lasers, Optical fibers, Ultraviolet radiation

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Proceedings Article | 10 March 1994 Paper

Fluorescence lifetime-based sensing of zinc in solution

Richard Thompson, Marcia Patchan

Proceedings Volume 2068, (1994) https://doi.org/10.1117/12.170676

KEYWORDS: Zinc, Luminescence, Phase measurement, Modulation, Metals, Chemical analysis, Sensors, Phase shifts, Demodulation, Biosensors

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Proceedings Article | 24 June 1993 Paper

Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging

Joseph Lakowicz, Henryk Szmacinski, Richard Thompson

Proceedings Volume 1895, (1993) https://doi.org/10.1117/12.146718

KEYWORDS: Modulation, Phase shift keying, Luminescence, Calcium, Glucose, Chemistry, Diagnostics, Energy transfer, Fluorescence lifetime imaging, Blood

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Proceedings Article | 18 May 1993 Paper

Homogeneous model immunoassay of thyroxine by phase-modulation fluorescense spectroscopy

Alvydas Ozinskas, Henryk Malak, Judy Britz, Richard Thompson, Peter Koen, Joseph Lakowicz

Proceedings Volume 1885, (1993) https://doi.org/10.1117/12.144697

KEYWORDS: Luminescence, Energy transfer, Phase shift keying, Modulation, Phase measurement, Sodium, Fluorescence resonance energy transfer, Blood, Polarization, Absorption

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Proceedings Article | 18 May 1993 Paper

Fiber optic ion sensors based on phase fluorescence lifetime measurements

Richard Thompson

Proceedings Volume 1885, (1993) https://doi.org/10.1117/12.144743

KEYWORDS: Luminescence, Fiber optics sensors, Phase measurement, Demodulation, Sensors, Chemical analysis, Modulation, Fiber optics, Phase shifts, Phase shift keying

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SPIE Journal Paper | 1 July 1992

Fluorometer and tapered fiber optic probes for sensing in the evanscent wave

Joel Golden, Lisa Shriver-Lake, George Anderson, Richard Thompson, Frances Ligler

OE, Vol. 31, Issue 07, (July 1992) https://doi.org/10.1117/12.10.1117/12.58764

KEYWORDS: Fiber optics, Optical filters, Biosensors, Connectors, Mirrors, Luminescence, Optical fibers, Signal generators, Fluorometers, Photodiodes

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Proceedings Article | 1 May 1990 Paper

Sensitivity enhancement for evanescent wave-excited fiber optic fluorescent sensors

Richard Thompson, Lynne Kondracki

Proceedings Volume 1204, (1990) https://doi.org/10.1117/12.17684

KEYWORDS: Fiber optics sensors, Luminescence, Waveguides, Fiber optics, Sensors, Optical fibers, Remote sensing, Refractive index, Cladding, Laser spectroscopy

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