Observation of the dynamic germination of single bacterial spores using rapid Raman imaging

Lingbo Kong; Peter Setlow; Yong-qing Li

doi:10.1117/1.JBO.19.1.011003

10 July 2013 Observation of the dynamic germination of single bacterial spores using rapid Raman imaging

Lingbo Kong, Peter Setlow, Yong-qing Li

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Journal of Biomedical Optics, Vol. 19, Issue 1, 011003 (July 2013). https://doi.org/10.1117/1.JBO.19.1.011003

Abstract

The dynamics of bacterial spore germination were successfully observed using a fast Raman imaging system, in combination with real-time phase contrast microscopy. By using a multifocus scan scheme, the spontaneous Raman-scattering imaging acquisition speed was increased to ∼30 s per frame while maintaining diffraction-limited resolution, which allowed monitoring of the dynamics of spore germination on a time scale of tens of seconds to a few minutes. This allowed simultaneous gathering of rich spatial distribution information on different cellular components including time-lapse molecular images of Ca-dipicolinic acid, protein, and nucleic acid during germination of single bacterial spores for the periods of 30 to 60 min.

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Lingbo Kong, Peter Setlow, and Yong-qing Li "Observation of the dynamic germination of single bacterial spores using rapid Raman imaging," Journal of Biomedical Optics 19(1), 011003 (10 July 2013). https://doi.org/10.1117/1.JBO.19.1.011003

Published: 10 July 2013

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