Production of silver nanoparticles (AgNPs) using different biological methods is gaining recognition due to their
multiple applications. Although, several physical and chemical methods have been used for the synthesis and
stabilizing of AgNPs, yet, a green chemistry method is preferable because it is cost effective and environmentally
friendly. The synthesis was done using Aloe Vera (AV) extract because it has chemical compounds such as
“Antrokinon” that are known for its antibacterial, antivirus and anticancer properties. We hypothesize that AV
extract can produce a stable nanoparticles within the 100 nm range and be biologically active. The biological
compounds were extracted from AV skin with water and ethanol which was used as the reduction agent for the
synthesis of nanoparticles. The biological extract and AgNO3 were blended and heated to synthesize AgNPs. The
reaction process was monitored using UV-Visible spectroscopy. Fourier Transfer Infrared spectroscopy (FTIR) was
used for the characterization of biological compounds and their substituent groups before and after the reaction
process. Dynamic Light scattering (DLS) method was used to characterize particle size of AgNPs and their biomolecular
stability. Results showed that biological compounds such as aliphatic amines, alkenes (=C-H), alkanes
(C-H), alcohol (O-H) and unsaturated esters(C-O), which has an average particle size of 109 and 215.8 nm and
polydispersity index of 0.451 and 0.375 for ethanol and water extract, respectively. According to TEM
measurements the size of AgNPs are in the range 5-20 nm The results suggested that ethanol derived AgNPs
contained higher yield of organic compounds, thus has better solubility power than water. Ag NPs can be used to
control salmonella in poultry industry.
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