Dr. Alain Brans Profile

Dr. Alain Brans

at Liège Univ.

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Proceedings Article | 5 March 2021 Presentation

Unexpected enhancement of Förster resonant energy transfer thanks to quantum dots aggregation

Julie Hottechamps, Thomas Noblet, Alain Brans, Christophe Humbert, Laurent Dreesen

Proceedings Volume 11659, 116590D (2021) https://doi.org/10.1117/12.2577023

KEYWORDS: Resonance energy transfer, Quantum dots, Molecules, Fluorescence resonance energy transfer, Semiconductors, Molecular energy transfer, Molecular aggregates, Luminescence, Energy transfer, Biosensors

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Here we investigate Förster Resonant Energy Transfer (FRET) occurring between luminescent colloidal semiconductor quantum dots (QDs) and fluorescent streptavidin molecules (FSA), chemically coupled thanks to N-hydroxysuccinimide (NHS) and biotin. In some conditions, QDs are known to form agglomerates throughout their functionalization by NHS and biotin molecules. Thus, we wondered how this collective aggregation could influence the efficiency of FRET. Interestingly, this proves to enhance the energy transfer from QDs to FSA. In terms of detection threshold, aggregated-QD-based systems lead to a limit down to 5 nM, while it is up to 80 nM for non-aggregated ones. Therefore, these unexpected results evidence our ability to exploit QDs aggregation for the design of biosensing systems with lower and lower molecular detection thresholds. Unlike the common beliefs, QD agglomeration is an asset that we can benefit from in order to improve the performance of QD-based biosensors. As a counterpart, this requires a fine monitoring of the emission spectrum of QDs while they are aggregating. This is why we provide a complete characterization of the QD fluorescence throughout their chemical funtionalization with NHS and biotin, supporting that such precautions are mandatory. Further, we show it is necessary to distinguish hetero-FRET (between QDs and FSA) from homo-FRET (between QDs within a same aggregate) in order to avoid misleading interpretations.

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