Ms. Anastasia D. Komarova Profile

Anastasia D. Komarova

at Privolzhsky Research Medical Univ

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Author

Publications (3)

SPIE Journal Paper | 30 September 2024 Open Access

Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm

Boris Yakimov, Anastasia Komarova, Elena Nikonova, Artem Mozherov, Liubov Shimolina, Marina Shirmanova, Wolfgang Becker, Evgeny Shirshin, Vladislav Shcheslavskiy

JBO, Vol. 29, Issue 10, 106501, (September 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.10.106501

KEYWORDS: Fluorescence, Fluorescence lifetime imaging, Nicotinamide adenine dinucleotide, Emission wavelengths, Mode conditioning cables, Sensors, Fluorescence intensity, Cancer, Microscopy, Tumors

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SignificanceAutofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for the evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights into the biochemical processes in the cells and biological tissues and facilitate the transition of spectral fluorescence lifetime imaging into clinical applications.AimWe present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under a single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.ApproachWe use two-photon spectral fluorescence lifetime imaging microscopy. Starting from model solutions, we switched to cell cultures treated by metabolic inhibitors and then studied the metabolism of cells within tumor spheroids.ResultsThe use of a multispectral detector in combination with an excitation at a single wavelength of 750 nm allows the identification of fluorescence signals from three components: free and bound NAD(P)H, and flavins based on the global fitting procedure. Multispectral data make it possible to assess not only the lifetime but also the spectral shifts of emission of flavins caused by chemical perturbations. Altogether, the informative parameters of the developed approach are the ratio of free and bound NAD(P)H amplitudes, the decay time of bound NAD(P)H, the amplitude of flavin fluorescence signal, the fluorescence decay time of flavins, and the spectral shift of the emission signal of flavins. Hence, with multispectral fluorescence lifetime imaging, we get five independent parameters, of which three are related to flavins.ConclusionsThe approach to probe the metabolic state of cells in culture and spheroids using excitation at a single wavelength of 750 nm and a fluorescence time-resolved spectral detection with the consequent global analysis of the data not only simplifies image acquisition protocol but also allows to disentangle the impacts of free and bound NAD(P)H, and flavin components evaluate changes in their fluorescence parameters (emission spectra and fluorescence lifetime) upon treating cells with metabolic inhibitors and sense metabolic heterogeneity within 3D tumor spheroids.

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Proceedings Article | 4 March 2022 Poster + Presentation + Paper

Nonlinear optical microscopy assessment of connective tissue changes in vulvar lichen sclerosus

Arseniy Potapov, Marina Sirotkina, Varvara Dudenkova, Vadim Elagin, Sergey Kuznetsov, Maria Karabut, Anastasia Komarova, Irina Kuznetsova, Stefka Radenska- Lppovok, Natalya Gladkova

Proceedings Volume 11965, 119650I (2022) https://doi.org/10.1117/12.2610241

KEYWORDS: Microscopy, Collagen, Second-harmonic generation, Connective tissue, Optical microscopy, Visualization, Homogenization, Skin, Tissues, Multiphoton microscopy

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Proceedings Article | 7 December 2021 Paper

Fluorescence and optoacoustics monitoring of tumor treatment with novel agents for combined photodynamic and chemotherapy

I. Turchin, M. Kirillin, A. Orlova, V. Perekatova, V. Plekhanov, E. Sergeeva, D. Kurakina, A. Khilov, A. Kurnikov, P. Subochev, M. Shirmanova, A. Komarova, D. Yuzhakova, A. Gavrina, S. Bano, S. Mallidi, T. Hasan

Proceedings Volume 11919, 119191J (2021) https://doi.org/10.1117/12.2614869

KEYWORDS: Tumors, Photodynamic therapy, Luminescence, Optoacoustics, IRIS Consortium, Tissues, Picosecond phenomena, Laser irradiation, Cancer, Reconstruction algorithms

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