Dr. Andrew York Profile

Dr. Andrew York

at Calico Life Sciences LLC

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Proceedings Article | 13 March 2024 Presentation

Magnetic control of GFP-like fluorescent proteins

Maria Ingaramo, Rebecca Frank Hayward, Julia Lazzari-Dean, Andrew York

Proceedings Volume PC12863, PC128630U (2024) https://doi.org/10.1117/12.3004017

KEYWORDS: Fluorescent proteins, Magnetism, Multiplexing, Magnetic resonance imaging, In vitro testing

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We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetoresponsive in the presence of an appropriate cofactor (e.g. EGFP-FlavinTag, or an mScarlet/FMN solution). This method works at room-temperature and body-temperature, in vitro, in E. coli and in cultured mammalian cells. The GFP-family magnetoresponse is weak (ΔF/F≈1%), but shows the hallmarks of evolvability. This suggests exciting technological possibilities, both short-term (e.g. lock-in detection, multiplexing) and long-term (e.g. optically-detected MRI, magnetogenetics). We've also discovered weak magnetoresponse from a member of the LOV-domain family. This suggests the possibility that magnetoresponse is a general feature of fluorescent proteins, and not unique to the cryptochrome/photolyase family.

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